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Merck KGaA magna meriptm m 6 a kit #17–10,499
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Merck KGaA merip m 6 a kit
Merip M 6 A Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA methylated rna immunoprecipitation kit guidelines (merip) m 6 a kit
A The predicted sites in S1PR3 mRNA in the SRAMP database. B Enrichment of m 6 A modification on S1PR3 as detected by <t>MeRIP-PCR</t> assay. C S1PR3 were elevated in 21 (87.5%) RCC tissues relative to adjacent nonmalignant tissues. D Relative luciferase activities of S1PR3-WT of S1PR3-MUT in WTAP KO 786-O cells with IGF2BPs knockdown. E The protein levels of S1PR3 and PI3K/AKT pathway following S1PR3 knockdown. F , G Knockdown of S1PR3 markedly suppressed migration and proliferation viabilities of RCC cells. Scale bar 250 μm. H Knockout of WTAP and IGF2BPs regulate PI3K/AKT pathway. I Schematic diagram of IGF2BP-mediated regulation of m 6 A-modified S1PR3 mRNA. Values in B and D are mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
Methylated Rna Immunoprecipitation Kit Guidelines (Merip) M 6 A Kit, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Zymo Research oligo clean concentrator kit
A The predicted sites in S1PR3 mRNA in the SRAMP database. B Enrichment of m 6 A modification on S1PR3 as detected by <t>MeRIP-PCR</t> assay. C S1PR3 were elevated in 21 (87.5%) RCC tissues relative to adjacent nonmalignant tissues. D Relative luciferase activities of S1PR3-WT of S1PR3-MUT in WTAP KO 786-O cells with IGF2BPs knockdown. E The protein levels of S1PR3 and PI3K/AKT pathway following S1PR3 knockdown. F , G Knockdown of S1PR3 markedly suppressed migration and proliferation viabilities of RCC cells. Scale bar 250 μm. H Knockout of WTAP and IGF2BPs regulate PI3K/AKT pathway. I Schematic diagram of IGF2BP-mediated regulation of m 6 A-modified S1PR3 mRNA. Values in B and D are mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
Oligo Clean Concentrator Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Abcam rna methylation quantification kit
A The predicted sites in S1PR3 mRNA in the SRAMP database. B Enrichment of m 6 A modification on S1PR3 as detected by <t>MeRIP-PCR</t> assay. C S1PR3 were elevated in 21 (87.5%) RCC tissues relative to adjacent nonmalignant tissues. D Relative luciferase activities of S1PR3-WT of S1PR3-MUT in WTAP KO 786-O cells with IGF2BPs knockdown. E The protein levels of S1PR3 and PI3K/AKT pathway following S1PR3 knockdown. F , G Knockdown of S1PR3 markedly suppressed migration and proliferation viabilities of RCC cells. Scale bar 250 μm. H Knockout of WTAP and IGF2BPs regulate PI3K/AKT pathway. I Schematic diagram of IGF2BP-mediated regulation of m 6 A-modified S1PR3 mRNA. Values in B and D are mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
Rna Methylation Quantification Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ribobio co merip m 6 a kit c11051-1
A The predicted sites in S1PR3 mRNA in the SRAMP database. B Enrichment of m 6 A modification on S1PR3 as detected by <t>MeRIP-PCR</t> assay. C S1PR3 were elevated in 21 (87.5%) RCC tissues relative to adjacent nonmalignant tissues. D Relative luciferase activities of S1PR3-WT of S1PR3-MUT in WTAP KO 786-O cells with IGF2BPs knockdown. E The protein levels of S1PR3 and PI3K/AKT pathway following S1PR3 knockdown. F , G Knockdown of S1PR3 markedly suppressed migration and proliferation viabilities of RCC cells. Scale bar 250 μm. H Knockout of WTAP and IGF2BPs regulate PI3K/AKT pathway. I Schematic diagram of IGF2BP-mediated regulation of m 6 A-modified S1PR3 mRNA. Values in B and D are mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
Merip M 6 A Kit C11051 1, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A The predicted sites in S1PR3 mRNA in the SRAMP database. B Enrichment of m 6 A modification on S1PR3 as detected by MeRIP-PCR assay. C S1PR3 were elevated in 21 (87.5%) RCC tissues relative to adjacent nonmalignant tissues. D Relative luciferase activities of S1PR3-WT of S1PR3-MUT in WTAP KO 786-O cells with IGF2BPs knockdown. E The protein levels of S1PR3 and PI3K/AKT pathway following S1PR3 knockdown. F , G Knockdown of S1PR3 markedly suppressed migration and proliferation viabilities of RCC cells. Scale bar 250 μm. H Knockout of WTAP and IGF2BPs regulate PI3K/AKT pathway. I Schematic diagram of IGF2BP-mediated regulation of m 6 A-modified S1PR3 mRNA. Values in B and D are mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.

Journal: Cell Death & Disease

Article Title: EGR2-mediated regulation of m 6 A reader IGF2BP proteins drive RCC tumorigenesis and metastasis via enhancing S1PR3 mRNA stabilization

doi: 10.1038/s41419-021-04038-3

Figure Lengend Snippet: A The predicted sites in S1PR3 mRNA in the SRAMP database. B Enrichment of m 6 A modification on S1PR3 as detected by MeRIP-PCR assay. C S1PR3 were elevated in 21 (87.5%) RCC tissues relative to adjacent nonmalignant tissues. D Relative luciferase activities of S1PR3-WT of S1PR3-MUT in WTAP KO 786-O cells with IGF2BPs knockdown. E The protein levels of S1PR3 and PI3K/AKT pathway following S1PR3 knockdown. F , G Knockdown of S1PR3 markedly suppressed migration and proliferation viabilities of RCC cells. Scale bar 250 μm. H Knockout of WTAP and IGF2BPs regulate PI3K/AKT pathway. I Schematic diagram of IGF2BP-mediated regulation of m 6 A-modified S1PR3 mRNA. Values in B and D are mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The MeRIP-quantitative real-time PCR was performed as per Magna methylated RNA immunoprecipitation kit guidelines (MeRIP) m 6 A Kit (Merck Millipore).

Techniques: Modification, Luciferase, Knockdown, Migration, Knock-Out