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Image Search Results
Journal: Cell Death & Disease
Article Title: EGR2-mediated regulation of m 6 A reader IGF2BP proteins drive RCC tumorigenesis and metastasis via enhancing S1PR3 mRNA stabilization
doi: 10.1038/s41419-021-04038-3
Figure Lengend Snippet: A The predicted sites in S1PR3 mRNA in the SRAMP database. B Enrichment of m 6 A modification on S1PR3 as detected by MeRIP-PCR assay. C S1PR3 were elevated in 21 (87.5%) RCC tissues relative to adjacent nonmalignant tissues. D Relative luciferase activities of S1PR3-WT of S1PR3-MUT in WTAP KO 786-O cells with IGF2BPs knockdown. E The protein levels of S1PR3 and PI3K/AKT pathway following S1PR3 knockdown. F , G Knockdown of S1PR3 markedly suppressed migration and proliferation viabilities of RCC cells. Scale bar 250 μm. H Knockout of WTAP and IGF2BPs regulate PI3K/AKT pathway. I Schematic diagram of IGF2BP-mediated regulation of m 6 A-modified S1PR3 mRNA. Values in B and D are mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.001.
Article Snippet: The MeRIP-quantitative real-time PCR was performed as per
Techniques: Modification, Luciferase, Knockdown, Migration, Knock-Out